Invitrogen™ CD49e (Integrin alpha 5) Monoclonal Antibody (eBioHMa5-1 (HMa5-1)), eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Supplier:  Invitrogen™ 14049385

Catalog No. 50-126-30

Description

Description: The eBioHMalpha5-1 monoclonal antibody reacts with mouse and rat CD49e, also known as integrin alpha5 or VLA-5A. CD49e is a member of the integrin alpha chain family, and binds with CD29 (integrin beta1) to form the receptor VLA-5, which binds the ligands fibronectin and fibrinogen. CD49e is a type I, single-pass membrane protein, with a molecular weight of 135 kDa, and is expressed on multiple cell types including thymocytes, mast cells, activated T cells and splenic B cells. In addition to its role in adhesion, VLA-5 contributes to T-cell co-stimulation. The eBioHMalpha5-1 monoclonal antibody has been demonstrated to interfere with VLA-5-mediated cell adhesion, and with the degranulation of IgE-sensitized rat RBL cells stimulated on fibronectin-coated plates. Furthermore, HMalpha5-1 injected into mice subcutaneously, along with anti-CD49d and anti-CD61, is able to inhibit passive cutaneous anaphylaxis. Applications Reported: This eBioHMa5-1 (HMa5-1) antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. (Please use Functional Grade purified eBioHMa5-1 (HMa5-1), cat. 16-0493, in functional assays.). Applications Tested: This eBioHMa5-1 (HMa5-1) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Specifications

• Antigen: CD49e (Integrin alpha 5)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P11688
• Gene Symbols: Itga5
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16402, 315346
• Content And Storage: 4°C
• Form: Liquid

• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation
• Clone: eBioHMa5-1 (HMa5-1)
• Conjugate: Unconjugated
• Gene: Itga5
• Gene Alias: 2-phospho-D-glycerate hydro-lyase; alpha enolase like 1; Alpha-enolase; alpha-enolase; c-myc promoter-binding protein-1; CD49 antigen-like family member E; Cd49e; C-myc promoter-binding protein; ENO1; ENO1L1; Enolase 1; enolase 1, (alpha); enolase-alpha; epididymis secretory protein Li 17; fibronectin receptor alpha polypeptide; fibronectin receptor subunit alpha; fibronectin receptor, alpha polypeptide; fibronectin receptor, alpha subunit; FNRA; HEL-S-17; integrin alpha 5; integrin alpha 5 (fibronectin receptor alpha); integrin alpha-5; Integrin alpha-5 heavy chain; Integrin alpha-5 light chain; Integrin alpha-F; integrin subunit alpha 5; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; Itga5_retired; MBP-1; MBPB1; MPB1; MPB-1; MYC promoter-binding protein 1; NNE; Non-neural enolase; phosphopyruvate hydratase; Plasminogen-binding protein; PPH; tau-crystallin; very late activation protein 5, alpha subunit; VLA5; VLA-5; VLA5A
• Host Species: Armenian Hamster
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

For Research Use Only.