Invitrogen™ CD49e (Integrin alpha 5) Monoclonal Antibody (eBioSAM-1 (SAM-1, SAM1)), Biotin, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 13049680

Catalog No. 50-115-81

Description

Description: The eBioSAM-1 monoclonal antibody reacts with human integrin alpha 5, also known as fibronectin receptor alpha chain, very late activation antigen 5 alpha, and CD49e. Integrins are composed of an alpha chain and a beta chain, which non-covalently associate to form the functional integrin. Integrin heterodimers participate in cell surface-mediated signaling and adhesion functions. Integrin alpha 5 undergoes post-translational cleavage in its extracellular domain to yield disulfide linked light and heavy chains that join with Integrin beta 1 (CD29) to form the fibronectin receptor, also known as the very late activation antigen-5 (VLA-5) complex. Integrin alpha 5 is expressed on thymocytes, T cells, monocytes, platelets, early B cells, and activated B cells. Applications Reported: This eBioSAM-1 (SAM-1, SAM1) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioSAM-1 (SAM-1, SAM1) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Specifications

• Antigen: CD49e (Integrin alpha 5)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P08648
• Gene Symbols: Itga5
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3678
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: eBioSAM-1 (SAM-1, SAM1), SAM1)
• Conjugate: Biotin
• Gene: Itga5
• Gene Alias: 2-phospho-D-glycerate hydro-lyase; alpha enolase like 1; Alpha-enolase; alpha-enolase; c-myc promoter-binding protein-1; CD49 antigen-like family member E; Cd49e; C-myc promoter-binding protein; ENO1; ENO1L1; Enolase 1; enolase 1, (alpha); enolase-alpha; epididymis secretory protein Li 17; fibronectin receptor alpha polypeptide; fibronectin receptor subunit alpha; fibronectin receptor, alpha polypeptide; fibronectin receptor, alpha subunit; FNRA; HEL-S-17; integrin alpha 5; integrin alpha 5 (fibronectin receptor alpha); integrin alpha-5; Integrin alpha-5 heavy chain; Integrin alpha-5 light chain; Integrin alpha-F; integrin subunit alpha 5; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; Itga5_retired; MBP-1; MBPB1; MPB1; MPB-1; MYC promoter-binding protein 1; NNE; Non-neural enolase; phosphopyruvate hydratase; Plasminogen-binding protein; PPH; tau-crystallin; very late activation protein 5, alpha subunit; VLA5; VLA-5; VLA5A
• Host Species: Mouse
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b