Invitrogen™ CD56 (NCAM) Monoclonal Antibody (TULY56), Brilliant Ultra Violet™ 563, eBioscience™

Catalog No.	Quantity
36-505-6641	25 Tests
36-505-6642	100 Tests

Catalog No. 36-505-6641 Supplier Invitrogen™ Supplier No. 365056641

Description

Mouse Monoclonal Antibody

This TULY56 monoclonal antibody reacts with human CD56, also known as Neural Cell Adhesion Molecule (NCAM). Staining with TULY56 does not block binding of CMSSB, suggesting that the two antibodies recognize different epitopes. Additionally, TULY56 performs better after fixation and permeabilization than CMSSB. The TULY56 monoclonal antibody crossreacts with Rhesus macaque. Applications Tested This TULY56 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Blocking Buffers When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information. Light sensitivity This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation Samples can be stored in IC Fixation Buffer (P...

CD56, also known as neural cell adhesion molecule (NCAM), is a highly glycosylated transmembrane glycoprotein of the immunoglobulin family. It plays a crucial role in cell adhesion, migration, axonal growth, pathfinding, and synaptic plasticity. CD56 is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa and is involved in homotypic adhesion of neural cells. It mediates interactions by binding extracellular matrix components such as laminin and integrins, with polysialic modification reducing CD56-mediated adhesion. In the hematopoietic system, CD56 is expressed on natural killer (NK) cells and a subset of T cells known as NKT cells. It is also found on most neuroectodermal-derived cell lines, tissues, and neoplasms, including retinoblastoma, medulloblastoma, astrocytomas, and neuroblastoma. CD56 serves as a widely used neuroendocrine marker with high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms, highlighting its importance in both neural and immune system functions.

Specifications

• Antigen: CD56 (NCAM)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: TULY56
• Concentration: 5 μL/Test
• Conjugate: Brilliant Ultraviolet 563
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: Ncam1
• Gene Accession No.: P13591
• Gene Alias: adhesion molecule; antigen recognized by monoclonal antibody 5.1H11; Cd56; CD-56; CD56 120 kDa GPI-linked isoform; CD56 140 kDa isoform; CD56 140 kDa VASE isoform; E NCAM; E-NCAM; MSK39; N CAM1; NCAM; N-CAM; Ncam1; N-CAM-1; NCAM-1; NCAMC; NCAM-C; neural cell adhesion molecule; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; sCD56; sNCAM; soluble CD56; soluble NCAM
• Gene Symbols: Ncam1
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 25 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 4684, 693789
• Target Species: Human, Monkey, Rhesus Macaque
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ