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CD6 Rat anti-Mouse, Clone: IM348, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

$63.50 - $107.00

Specifications

Antigen CD6
Clone IM348
Target Species Mouse
Conjugate Unconjugated
Isotype IgG2a
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-123-81 Non-distribution product as customer accommodation.
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Invitrogen
14006180
25 μg Each for $63.50
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50-123-82 Non-distribution product as customer accommodation.
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Invitrogen
14006182
100 μg Each for $107.00
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Description

Description

Description: The monoclonal antibody IM348 recognizes mouse CD6, a transmembrane glycoprotein of the scavenger receptor family. The extracellular domain contains three scavenger receptor cysteine rich (SRCR) domains, a transmembrane region and a cytoplasmic domain of variable length depending on the isoform. CD6 is expressed on most T lymphocytes. Expression between human and mouse is slightly different. In mice B cells in the spleen do not have CD6, while humans express it on a subset of B lymphocytes. The CD6 ligand is CD166, also known as Activated Leukocyte Cell Adhesion Molecule (ALCAM). Through interactions with CD166 or homotypic CD6-CD6, a role exists in adhesion and migration during organogenesis. The intracellular domain of CD6 is constitutively phosphorylated, suggesting a role in T cell activation and signaling. In humans depletion of CD6-expressing cells increases graft versus host tolerance in transplants. Applications Reported: This IM348 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistology staining of frozen tissue sections. Applications Tested: This IM348 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The IM348 antibody can also be used at <5 µg/mL in immunoblotting of non-reduced lysates. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. This gene may be associated with susceptibility to multiple sclerosis (PMID: 19525953, 21849685). Multiple transcript variants encoding different isoforms have been found for this gene.
Specifications

Specifications

CD6
Mouse
IgG2a
Monoclonal
Liquid
Antibody
4° C
IM348
Unconjugated
Flow Cytometry, Immunohistochemistry (Frozen), Western Blot
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.

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