Invitrogen™ CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), PE, eBioscience™, Invitrogen™

Catalog No.	Quantity
50-112-9536	50 μg
50-104-36	100 μg
50-104-37	200 μg

Catalog No. 50-112-9536 Supplier Invitrogen™ Supplier No. 12061181

Description

Armenian Hamster Monoclonal Antibody

Description: The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin alpha subunits; alphaV (CD51) to form Vitronectin Receptor and alphaIib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor. Applications Reported: The 2C9.G3 antibody has been reported for use in flow cytometric analysis. Applications Tested: The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD61, also known as GPIIIa or ITGB3, is a 105 kDa glycoprotein expressed on activated T cells, granulocytes, megakaryocytes, platelets, and their precursors. It plays a crucial role in platelet aggregation and functions as a receptor for fibrinogen, fibronectin, von Willebrand factor, vitronectin, and thrombospondin. CD61 forms heterodimeric complexes by associating non-covalently with integrin alpha subunits: alphaV (CD51) to create the Vitronectin Receptor and alphaIIb (CD41) to form gpIIb/IIIa. These complexes are responsible for adhesion to extracellular matrix components, facilitating cell adhesion and cell-surface mediated signaling. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, and osteoclasts in association with CD51. The protein product of CD61 is composed of an alpha chain and a beta chain, which can combine with multiple partners to form different integrins. Its involvement in cell adhesion and signaling underscores its importance in normal physiological processes. Dysfunction of CD61 is associated with diseases such as Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder, highlighting its critical role in hemostasis and platelet function.

Specifications

• Antigen: CD61 (Integrin beta 3)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 2C9.G3
• Concentration: 0.2 mg/mL
• Conjugate: PE
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: ITGB3
• Gene Accession No.: O54890
• Gene Alias: antigen CD61; BDPLT16; BDPLT2; beta3 integrin; CD61; EGK_08849; glycoprotein GPIIIa; glycoprotein IIIa; GP3A; GPIIIa; GT; INGRB3; integrin beta 3; integrin beta 3 (Cd61); integrin beta-3; integrin beta-3 subunit; integrin subunit beta 3; integrin, beta 3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); ITGB3; platelet glycoprotein IIIa; platelet glycoprotein IIIa integrin subunit beta 3; platelet gpIIIa; platelet membrane glycoprotein IIIa
• Gene Symbols: ITGB3
• Host Species: Armenian Hamster
• Purification Method: Affinity chromatography
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 16416, 29302
• Target Species: Mouse, Rat
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

For Research Use Only.