CD62P (P-Selectin) Mouse anti-Mouse, Functional Grade, Clone: Psel.KO2.12, eBioscience™ 

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 16062285

Catalog No. 50-143-66

Description

Description: CD62P (also known as P-selectin and GMP-140) is a 140-kDa cell adhesion molecule that can be induced on endothelial cells and platelets. During inflammation, this selectin mediates adhesion of monocytes and neutrophils to activated platelets, as well as leukocyte rolling on endothelial cells. The major ligand for CD62P on neutrophils is P-selectin glycoprotein ligand-1 (PSGL-1). This monoclonal antibody recognizes an epitope that is conserved across several species (e.g. mouse, human, and rat). Applications Reported: This Psel.KO2.12 antibody has been reported for use in flow cytometric analysis and for blocking CD62P-mediated adhesion. Applications Tested: This Psel.KO2.12 antibody has been tested by flow cytometric analysis on thrombin-activated mouse platelets. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.

001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC. P-selectin is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate.

Specifications

• Antigen: CD62P (P-Selectin)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: Q01102
• Gene Alias: RP1-86F14.2, CD62, CD62P, FLJ45155, GMP140, GRMP, LECAM3, PADGEM, PSEL
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Mouse

• Applications: Flow Cytometry, Functional Assay, Neutralization
• Clone: Psel.KO2.12
• Conjugate: Functional Grade
• Gene: Selp
• Format: Liquid
• Gene Symbols: Selp
• Isotype: IgG1
• Quantity: 500 μg
• Storage Requirements: 4° C
• Primary or Secondary: Primary
• Gene ID (Entrez): 20344

For Research Use Only.