CD62P Rat anti-Mouse, BB700, Clone: RB40.34, BD Optibuild™

Catalog No.	Quantity
BDB742128	50 μg

Catalog No. BDB742128 Supplier BD Biosciences Supplier No. 742128

Description

Rat Monoclonal Antibody

The RB40.34 monoclonal antibody specifically binds to mouse P-selectin (CD62P), a 140 kDa protein which is expressed on activated platelets, activated endothelial cells, and megakaryocytes. P-selectin mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells, mediates leukocyte rolling, and is involved in the migration of leukocytes into inflamed tissues. CD24 and CD162 (PSGL-1) are ligands of CD62P. mAb RB40.34 can block mouse P-selectin binding to its ligands in vitro and in vivo.

The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes. It is a polymer-based tandem dye developed exclusively by BD Biosciences. With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40nm filter). This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.BD Horizon Brilliant™ Blue 700 (BB700) is a dye that was exclusively developed by BD Biosciences as brighter alternative to PerCP-Cy5.5. This dye also has less cross laser excitation off the 405 nm laser, resulting in less spillover into the violet channels compared to PerCP-Cy5.5.  Due to similar excitation and emission properties, BD Horizon BB700 and PerCP-Cy5.5 cannot be used simultaneously.

Specifications

• Antigen: CD62P
• Classification: Monoclonal
• Clone: RB40.34
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Blue 700 (BB700)
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: P-selectin, Selp, LECAM3, LYAM3, PADGEM, GMP-140, Grmp
• Host Species: Rat
• Immunogen: P-selectin-IgG1 Fusion
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 λ