Novus Biologicals™ CD8 Antibody Pair [HRP]

Description

Polyclonal/Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Mouse CD8.

Specifications

• Assay Range: 46.9 pg/mL
• Assay Sensitivity: 46.88-3000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Mouse CD8.
• Target Species: Mouse
• For Use With (Application): Sandwich ELISA
• Gene Alias: CD8, CD8a molecule, LEU2, p32
• Gene ID (Entrez): 925
• Gene Symbol: CD8A
• Host Species: Rabbit
• Kit Contents:
  Standard: Each vial contains 40 ng of recombinant mouse CD8.
  Capture Antibody: 0.5 mg/mL of rabbit anti-mouse CD8 monoclonal antibody.
  Detection Antibody: 0.4 mg/mL rabbit anti-CD8 polyclonal antibody conjugated to horseradish-peroxidase (HRP).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Adaptive Immunity, Cytokine Research, Immunology, Innate Immunity, Phospho Specific, Signal Transduction, Stem Cell Markers
• Storage Requirements: Storage is content dependent.
• Target: CD8
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in CBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended.