CD80 (B7-1) Monoclonal Antibody (2D10.4), PE-Cyanine5, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-140-77	Invitrogen 15080942	100 Tests

Description

Description: The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively. Applications Reported: The 2D10.4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 2D10.4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

Specifications

• Antigen: CD80 (B7-1)
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P33681
• Gene Symbols: CD80
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: CD80

• Clone: 2D10.4
• Applications: Flow Cytometry
• Conjugate: PE-Cyanine5
• Host Species: Mouse
• Target Species: Human
• Gene ID (Entrez): 941
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.