Novus Biologicals™ CD82/Kai-1 Antibody Pair [HRP]

Description

Rabbit Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human CD82/Kai-1.

Specifications

• Assay Range: 78 pg/mL
• Assay Sensitivity: 78.13-5000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human CD82/Kai-1.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: CD82 antigenC33, CD82 molecule, IA4C33 antigen, Inducible membrane protein R2, KAI1GR15, kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyteantigen, antigen detected by monoclonal and IA4)), Metastasis suppressor Kangai-1, R2, SAR2tetraspanin-27, ST6tspan-27, Suppressor of tumorigenicity 6 protein, Tetraspanin-27, Tspan-27, TSPAN274F9
• Gene ID (Entrez): 3732
• Gene Symbol: CD82
• Host Species: Rabbit
• Kit Contents:
  Standard: Each vial contains 47 ng of recombinant CD82/Kai-1.
  Capture Antibody: 0.5 mg/mL of rabbit anti-CD82/Kai-1 monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.17 mg/mL rabbit anti-CD82/Kai-1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 67 % HRP-Protector, pH 7.4).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cancer
• Storage Requirements: Storage is content dependent.
• Target: CD82/Kai-1
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in CBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5 ng/mL is recommended.