Invitrogen™ CD85k (Gp49b) Monoclonal Antibody (H1.1), Functional Grade, eBioscience™

Catalog No.	Quantity
50-245-626	500 μg

Catalog No. 50-245-626 Supplier Invitrogen™ Supplier No. 16578485

Description

Armenian Hamster Monoclonal Antibody

The monoclonal antibody H1.1 recognizes gp49, a receptor with structural similarities to the PIR (paired Ig-activating receptors) and ILT/KIR(killer inhibitory receptors) family of proteins. Two closely linked genes on mouse chromosome 10 encode the related proteins gp49A and gp49B. These two receptors are almost 90% identical in their extracellular regions but differ in their cytoplasmic domains; gp49B contains an ITAM domain while gp49A does not and is extremely short. Expression of gp49 was originally identified on mast and LAK (IL-2 activated NK) cells. Expression on LAK cells has been shown to be predominantly gp49B with only a small amount of gp49A. Additionally gp49 is found on myeloid cells. Detailed function is not known but gp49B can inhibit cellular activation. The monoclonal antibody H1.1 recognizes both gp49A and gp49B. The H1.1 antibody has been shown to have functional activity: when crosslinked along with anti-NK1.1 it can prevent cytokine release in KY-2 cells. Applications Reported: The H1.1 antibody has been reported for use in flow cytometric analysis and functional assays. It has been reported to block cytokine release. Applications Tested: This H1.1 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empiri...

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. The receptor can also function in antigen capture and presentation. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

Specifications

• Antigen: CD85k (Gp49b)
• Applications: Functional Assay, Neutralization
• Classification: Monoclonal
• Clone: H1.1
• Concentration: 1 mg/mL
• Conjugate: Functional Grade
• Formulation: PBS with no preservative; pH 7.2
• Gene: Lilrb4a
• Gene Accession No.: Q64281
• Gene Alias: CD85 antigen-like family member K; CD85K; glycoprotein 49 B; glycoprotein 49b; gp49; Gp49b; GP49B2; HM18; ILT3; ILT-3; Immunoglobulin-like transcript 3; leucocyte Ig-like receptor B4; leukocyte immunoglobulin like receptor B4; leukocyte immunoglobulin-like receptor 5; Leukocyte immunoglobulin-like receptor subfamily B member 4; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4; leukocyte immunoglobulin-like receptor, subfamily B, member 4; leukocyte surface receptor; Lilrb4; LIR5; LIR-5; mast cell surface glycoprotein Gp49B; Monocyte inhibitory receptor HM18
• Gene Symbols: Lilrb4a
• Host Species: Armenian Hamster
• Purification Method: Affinity chromatography
• Quantity: 500 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 14728
• Target Species: Mouse
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG