CD88 (C5aR1) Monoclonal Antibody (20/70), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
12-088-282	Invitrogen™ 12088282	100 μg

Description

Description: This 20/70 monoclonal antibody recognizes mouse CD88 also known as C5aR1. CD88 is a complement receptor widely expressed on cells of the myeloid lineage. Clone 20/70 has been reported to block the binding of C5a to murine CD88. Applications Reported: This 20/70 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 20/70 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

CD88, also known as C5aR1, is a C5a Anaphylatoxin Receptor that mediates the effects of one of the most potent chemotactic agents known. C5AR1 has been implicated in asthma, and its expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract. CD88 is the receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. Functionally, CD88 stimulates chemotaxis, granule enzyme release and superoxide anion production. CD88 is also a component of the classical pathway of the complement cascade; it may be involved with tissue injury associated with ischemia or sepsis and it may be a component of the membrane attack complex. Diseases associated with CD88 include Mannose-Binding Lectin Deficiency and Systemic Lupus Erythematosus.

Specifications

• Antigen: CD88 (C5aR1)
• Applications: Flow Cytometry
• Conjugate: PE
• Host Species: Rat
• Target Species: Mouse
• Gene ID (Entrez): 12273
• Isotype: IgG2b κ
• Purification Method: Affinity Chromatography
• Product Type: Antibody

• Clone: 20/70
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P30993
• Immunogen: RBL-2H3 transfected cells expressing murine C5aR.
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!