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  6. Invitrogen™ CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), Brilliant Ultra Violet™ 395, eBioscience™ 

Invitrogen™ CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), Brilliant Ultra Violet™ 395, eBioscience™

Catalog No. 363090080
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36-309-0080 25 μg
36-309-0082 100 μg
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Catalog No. 36-309-0080 Supplier Invitrogen™ Supplier No. 363090080
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Description

Mouse Monoclonal Antibody

The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflamed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.Applications TestedThe HIS51 antibody has been tested by flow cytometric analysis of rat thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.25 g per test. A test is defined as the amount (g) of antibody that will stain a cell sample in a final volume of 100 L. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Blocking BuffersWhen using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222)...

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD90.1 (Thy-1.1)
Applications Flow Cytometry
Classification Monoclonal
Clone HIS51
Concentration 0.2 mg/mL
Conjugate Brilliant Ultraviolet 395
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Thy1
Gene Accession No. P01830, P01831
Gene Alias CD7; CD90; FLJ33325; T25; Thy 1.2; Thy1; Thy-1; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 glycoprotein; thy-1 membrane glycoprotein; Thy-1 protein; Thy1.1; Thy1.2; Thy-1.2; thymus cell antigen 1, theta; Thymus cell surface antigen
Gene Symbols Thy1
Host Species Mouse
Purification Method Affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 21838, 24832
Target Species Mouse, Rat
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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