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  6. Invitrogen™ CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), PE-Cyanine7, eBioscience™, Invitrogen™ 

Invitrogen™ CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), PE-Cyanine7, eBioscience™, Invitrogen™
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Catalog No. 25090382
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25-090-382 100 μg
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Catalog No. 25-090-382 Supplier Invitrogen™ Supplier No. 25090382
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Description

Rat Monoclonal Antibody

Description: The 30-H12 monoclonal antibody reacts with mouse CD90.2, also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/J, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 30-H12. CD90 is involved in regulation of adhesion and signal transduction by T cells. Applications Reported: This 30-H12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 30-H12 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This may be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No.

CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.
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Specifications

Antigen CD90.2 (Thy-1.2)
Applications Flow Cytometry
Classification Monoclonal
Clone 30-H12
Concentration 0.2 mg/mL
Conjugate PE-Cyanine7
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Thy1
Gene Accession No. P01831
Gene Alias CD90; FLJ33325; T25; Thy 1.2; Thy1; Thy-1; Thy-1 antigen; thy-1 membrane glycoprotein; Thy1.1; Thy1.2; Thy-1.2; thymus cell antigen 1, theta
Gene Symbols Thy1
Host Species Rat
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 21838
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2b κ
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