CD95 Mouse anti-Baboon, Cynomolgus, Human, Porcine, Brilliant Ultraviolet 737, Clone: DX2, BD Horizon™

Catalog No.	Quantity
BDB612790	100 Tests

Catalog No. BDB612790 Supplier BD Biosciences Supplier No. 612790

Description

Mouse Monoclonal Antibody

The DX2 monoclonal antibody specifically binds to the human Fas antigen (also called APO-1). This 45 kDa transmembrane cell surface molecule was designated as CD95 at the Fifth HLDA Workshop. Fas is a member of the TNF-receptor superfamily and is also known as Tumor necrosis factor receptor superfamily member 6 (TNFRSF6). It is expressed on a variety of normal and neoplastic cells including activated T and B lymphocytes and some undifferentiated thymocytes. The Fas/CD95 antigen is a polypeptide that plays a role in the programmed sequence of events leading to cell death, termed apoptosis. The DX2 clone specifically reacts with murine L cells, murine L1210 leukemia cells and murine P815 mastocytoma cells transfected with human Fas cDNA but not with untransfected parental cell lines. Crosslinking CD95 with DX2 antibody delivers an apoptotic signal indicating that DX2 recognizes a functional epitope of the CD95 antigen.

The antibody was conjugated to BD Horizon BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm. BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor™ 700-like dyes (e.g., 712/20-nm filter).

Specifications

• Antigen: CD95
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: DX2
• Conjugate: Brilliant Ultraviolet 737
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Human CD95-transfected L Cells
• Purification Method: Affinity Purified
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Baboon, Cynomolgus, Human, Pig
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov