Invitrogen™ Cdc37 Monoclonal Antibody (C1)

Catalog No.	Quantity
MA3029	100 μL

Catalog No. MA3029 Supplier Invitrogen™ Supplier No. MA3029

Description

Mouse Monoclonal Antibody

MA3-029 detects Cdc37 from hamster, human, mouse and rat cells and tissues. MA3-029 has been successfully used in Western blot, FACS, immunofluorescence, immunocytochemistry and immunoprecipitation (under denaturing conditions) procedures. By Western blot, this antibody recognizes a single 50 kDa protein representing Cdc37 in HeLa cell extract. Immunofluorescence staining of Cdc37 in Hepa1 cells with MA3-029 results in mostly cytoplasmic staining concentrated around the nuclear membrane. This antibody detects some weak non-specific bands in Jurkat, A431 and NIH-3T3 cell lysates. The MA3-029 antigen is purified mouse Cdc37 fusion protein.

In S. cerevisiae, Cdc37 has been shown to be an important regulator of cell division cycle through its action on cyclin-dependent kinases (CDK's). Cdc37 has also been associated with signaling pathways and kinases unrelated to cell cycle control, including v-Src, casein kinase II, MPS1 kinase and the sevenless receptor tyrosine kinase. Interestingly, HSP90 has also been shown to be associated with many of the same cellular targets as Cdc37. It has now been shown that Cdc37 is the same protein previously described as p50 which has been found to be associated with many of the same proteins as HSP90. Among others, Cdc37/p50 has been found complexed with Src-family kinases (Fyn, Yes, Fes, Lck & Fgr), Raf and pp60v-src. More recently, Cdc37 has been shown to be a chaperone independently and in concert with HSP90. In fact, Cdc37 is necessary for the binding of HSP90 to Cdk4.

Specifications

• Antigen: Cdc37
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: C1
• Concentration: Conc. Not Determined
• Conjugate: Unconjugated
• Formulation: ascites with 0.05% sodium azide
• Gene: CDC37
• Gene Accession No.: Q16543, Q61081, Q63692
• Gene Alias: CDC 37; CDC37; CDC37 (cell division cycle 37 S. cerevisiae homolog); CDC37 (cell division cycle 37, S. cerevisiae, homolog); CDC37 cell division cycle 37 homolog; Cdc37 homolog; CDC37 protein; CDC37A; Cdc37p; Cell division control protein 37; cell division cycle 37; cell division cycle 37 homolog; hsp90 chaperone protein kinase-targeting subunit; Hsp90 co-chaperone Cdc37; Hsp90 co-chaperone Cdc37, N-terminally processed; p50; P50CDC37; SMO1; YD9489.03; YDR168W
• Gene Symbols: CDC37
• Host Species: Mouse
• Immunogen: Purified mouse Cdc37 fusion protein.
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100760178, 11140, 114562, 12539
• Target Species: Human, Mouse, Rat, Hamster
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov