Invitrogen™ CDK4 Monoclonal Antibody (DCS-31.2)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA182085

Catalog No. PIMA182085

Description

Mouse anti CDK4 antibody clone DCS31.2 recognizes cyclin dependent kinase 4 (Cdk4), a ~34 kDa protein involved in the regulation of cell cycle progression between G1 and S.Mouse anti CDK4 antibody clone DCS31.2 does not cross react with other cdk's and does not co-precipitate the D type cyclins bound to cdk4.

CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.

Specifications

• Antigen: CDK4
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Formulation: PBS with 0.08% sodium azide
• Gene Accession No.: P11802, P30285, P35426
• Gene Symbols: Cdk4
• Immunogen: Proprietary Immunogen.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
• Clone: DCS-31.2
• Conjugate: Unconjugated
• Gene: Cdk4
• Gene Alias: Cdk4; Cell division protein kinase 4; CMM3; Crk3; cyclin dependent kinase 4; cyclin-dependent kinase 4; MGC14458; p34>PSK-J3>/cdk4; PSK-J3; serine/threonine kinase; Unknown (protein for MGC:133903)
• Host Species: Mouse
• Purification Method: Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 1019, 12567, 94201
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid