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Invitrogen™ CDP-Star™ Substrate (0.4 mM Ready-to-Use) with Enhancer

Catalog No. T2216
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Emerald-II™ Enhancer
Sapphire-II™ Enhancer
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Catalog No. Description
T2216 Emerald-II™ Enhancer
T2214 Sapphire-II™ Enhancer
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Catalog No. T2216 Supplier Invitrogen™ Supplier No. T2216
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Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.

Chemiluminescent substrates provide highly sensitive replacements for the widely used fluorogenic substrate methylumbelliferyl phosphate (MUP), and the colorimetric substrate p-nitrophenyl phosphate (pNPP). Low background luminescence coupled with high-intensity light output enables detection of alkaline phosphatase labels with the highest possible sensitivity and signal-to-noise.

The CDP-Star™ Substrate with Sapphire-II™ or Emerald-II™ Enhancer is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.

This CDP-Star™ chemiluminescent substrate for alkaline phosphatase let you detect alkaline phosphatase and alkaline phosphatase-labeled molecules with unparalleled sensitivity, speed, and ease.

• Chemiluminescent substrates provide highly sensitive replacements for the widely used fluorogenic substrate methylumbelliferyl phosphate (MUP), and the colorimetric substrate p-nitrophenyl phosphate (pNPP).
• Low background luminescence coupled with high-intensity light output enables detection of alkaline phosphatase labels with the highest possible sensitivity and signal-to-noise.

This versatile chemiluminescent substrate exhibits high sensitivity in membrane-based applications such as Southern, Northern, and Western blotting. This substrate can also be used in solution-based assays such as immunoassays, DNA probe assays, enzyme assays, and reporter gene assays. Maximum light levels are reached in approximately 10 minutes and glow emission persists for several hours. Solution assays require chemiluminescent enhancer. Membrane-based assays may also require it for increased light output and sensitivity.

For Research Use Only. Not for use in diagnostics procedures.

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Specifications

Description Emerald-II™ Enhancer
Product Type ELISA Substrate with Enhancer
Quantity 100 mL
Detection Method Chemiluminescence
Form Liquid
Format Bottle
Product Line CDP-Star, NovaBright
Substrate Type Alkaline Phosphatase
Content And Storage Store the product at 4°C.
Shipping Condition Room Temperature
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