Invitrogen eBioscience Cell Stimulation Cocktail, 500x
Cocktail of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines.
Manufacturer: Invitrogen 00497003
- Can be used to induce cytokine production in in vitro cell culture for subsequent detection in immunoassays
- Can also be used in conjunction with the Protein Transport Inhibitor Cocktail (500X) (cat. 00-4980) for intracellular retention of the induced cytokine expression
|500X (Use at 1X: dilute 1/500 or to 2μL/mL final concentration)|
|Phorbol 12-Myristate 13-Acetate (40.5 μM), Ionomycin (670 μM) in Ethanol (500X)|
|−20°C. Protect from light.|
|Flow Cytometric Analysis, Functional Assays, Cell Culture, ELISA|
|4 x 100μL|
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