Molecular Probes™ CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

Description

The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells.

The kit includes the novel fluorogenic substrate CellEvent™ Caspase-3/7 Green Detection Reagent, as well as SYTOX™ AADvanced™ Dead Cell Stain.

• Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7
• Easy identification—clearly identify live, dead, and apoptotic cell populations
• Quick analysis—washing and fixation is not required
• Multicolor compatibility—combine with other dyes excitable by the 488 nm laser or other lasers

CellEvent™ Caspase-3/7 Green Detection Reagent is a cell-permeant reagent that consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorogenic signal that has absorption/emission maxima of ∼511/533 nm. When used together with the SYTOX™ AADvanced™ Dead Cell Stain, apoptotic cells can be easily discriminated from live and necrotic cells.

Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis.

Specifications

• Quantity: 100 Assays
• Format: Kit
• Product Type: Caspase Assay Kit
• Content And Storage: Contains 1 vial CellEvent™ Caspase 3/7 Green Detection Reagent, 1 vial SYTOX AADvanced dead cell stain, and 1 vial of DMSO.
  
  Store all vials in the kit upright, at or below -20deg;C, desiccated, and protected from light.
• Flow Cytometer Laser Lines: 488
• Excitation/Emission: SYTOX AADvanced: 546/647
  CellEvent Caspase 3/7 Green Detection Reagent: 511/533
• Shipping Condition: Wet Ice
• Solubility: DMSO (Dimethylsulfoxide)
• Product Line: CellEvent™
• Detection Method: Fluorescence
• For Use With (Application): Flow Cytometry
• For Use With (Equipment): Attune™ Acoustic Focusing Cytometer, Flow Cytometer
• Label or Dye: CellEvent™ Caspase 3/7 Green Detection Reagent, SYTOX AADvanced dead cell stain
• Label Type: Other Label(s) or Dye(s)

For Research Use Only. Not for use in diagnostic procedures.