Invitrogen™ Cells-to-CT™ Express 1-Step RT-qPCR Master Mix

Catalog No.	Quantity
A40012178	5 x 10 mL

Catalog No. A40012178 Supplier Invitrogen™ Supplier No. A40012178

Description

Cells-to-CT™ Express 1-Step RT-qPCR Master Mix is a bulk-format reagent that combines RT and qPCR into 1-step, simplifying direct-lysis workflows, reducing handling and variability, and supporting efficient automated and high-throughput applications.

Cells-to-CT™ Express 1-Step RT-qPCR Master Mix is a bulk-format solution designed to simplify and scale direct-lysis gene expression workflows. By combining reverse transcription and qPCR into a single reaction, it helps reduce hands-on steps and workflow variability, and improve operational efficiency. The large-volume format is well suited for automated and high-throughput laboratories seeking a streamlined Cells-to-CT™ workflow.

• Streamlined 1-step RT-qPCR workflow: Combines reverse transcription (RT) and quantitative PCR (qPCR) in a single reaction tube, reducing hands-on steps, workflow complexity, and potential sources of variability.
• Designed for direct-lysis workflows: Use with TaqMan™ Cells-to-CT™ Express Lysis Reagent enables crude lysate to be added directly to the RT-qPCR reaction, reducing the need for traditional nucleic acid purification.
• Reduced sample processing: Bypassing RNA purification helps decrease intermediate handling, reducing the potential for sample loss or degradation associated with extraction procedures.
• Lysate-compatible formulation: Optimized to support robust amplification in the presence of cellular debris and other components found in unpurified lysates, enabling an efficient transition from cell or tissue lysis to gene expression analysis.
• Supports high-throughput and automated workflows: The large-volume bulk format is well suited for research laboratories with high reagent demands, helping improve operational efficiency and simplify implementation in automated qPCR workflows.

Reduced procedural complexity: Fewer processing and reaction setup steps help minimize hands-on time and the potential for procedural error, making the workflow well suited for high-throughput and time-sensitive applications.

Specifications

• Concentration: 4X
• Detection Method: Primer-probe
• Form: Liquid
• GC-Rich PCR Performance: High
• PCR Method: 1-step RT-qPCR
• Reaction Speed: Fast or Standard
• Label or Dye: ROX
• Passive Reference Dye: Rox
• Product Type: RT-qPCR Master Mix
• Quantity: 5 x 10 mL
• Reverse Transcriptase: M-MLV
• For Use With (Application): Gene Expression, Direct PCR
• No. of Reactions: 10,000 x 20 μL Reactions
• Sample Type: Direct Cell Lysis

For Research Use Only. Not for use in diagnostic procedures.