Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. General Cell Based Assays
  6. K1817

Thermo Scientific™ CellSensor™ ESRE-bla HeLa Cell Line

Catalog No. K1817
Encompass_Preferred
Change view
Click to view available options
Quantity:
1 Vial
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
K1817 1 Vial
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. K1817 Supplier Thermo Scientific™ Supplier No. K1817
Only null left
Add to Cart
Add to Cart
Request a Quote

Description

Endoplasmic Reticulum (ER) stress is associated with a variety of pathophysiological conditions, such as neurodegenerative diseases, diabetes, tumor growth under hypoxic conditions, and ischemic heart disease.

Endoplasmic Reticulum (ER) stress is associated with a variety of pathophysiological conditions, such as neurodegenerative diseases, diabetes, tumor growth under hypoxic conditions, and ischemic heart disease. Proteins in the ER misfold or unfold, and accumulate under stress conditions, which promote the expression of ER stress responsive genes. One of the mechanisms for mediating ER stress response is the activation of transcription factor ATF6. The quiescent form of ATF6 (p90ATF6), a type II-transmembrane protein, is embedded in the ER membrane and proteolyzed in an ER stress-dependent manner. The liberated N-terminal fragment (p50ATF6) translocates to the nucleus, binding to ER stress response element (ERSE) present in the proximal promoter regions of many ER stress-responsive proteins including ER chaperones. To better understand the pathological processes and provide novel avenues to potential therapies, ESRE–bla HeLa cells are engineered to express beta-lactamase under the control of ER stress response element. This is a clonal population isolated by FACS and its dose response curves with tunicamycin and thapsigargin are performed. This cell line also response to other known ER stress inducers.

TRUSTED_SUSTAINABILITY

Specifications

Alternate Agonists Thapsigargin
Antagonists Salubrinal
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage CellSensor™ ESRE-bla HeLa cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Detection Method Fluorescence
For Use With (Application) FRET
Gene Symbol ESRE
Human Protein Class Cell Signalling
Primary Agonist Tunicamycin
Product Line CellSensor
Quantity 1 Vial
Readout End Point
Reporter Gene BLA (Beta-Lactamase), BLA (Beta-lactamase)
Target Entry ATF6, ER stress response pathway
Cell Line HeLa
System Type CellSensor™
Druggable Target Signaling Pathway
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.