Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. General Cell Based Assays
  6. K1644

Thermo Scientific™ CellSensor™ HRE-bla ME-180 Cell Line

Catalog No. K1644
Encompass_Preferred
Change view
Click to view available options
Quantity:
1 Vial
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
K1644 1 Vial
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. K1644 Supplier Thermo Scientific™ Supplier No. K1644

Please call customer service for current pricing. 1-800-766-7000

Description

The CellSensor™ HRE-bla ME-180 cell line contains a beta-lactamase reporter gene under control of the hypoxia response element (HRE) stably integrated into ME-180 cells.

The CellSensor™ HRE-bla ME-180 cell line contains a beta-lactamase reporter gene under control of the hypoxia response element (HRE) stably integrated into ME-180 cells. To construct this cell line, the HRE-bla construct was transduced into ME-180 cells by lentivirus. Flow cytometry was used to isolate cells responsive to deferoxamine. This cell line is validated for DMSO tolerance, cell number, stimulation time, substrate loading time, as well as Z' and EC50 concentrations for cobalt chloride and deferoxamine. The CellSensor™ HRE-bla ME-180 cell line is responsive to deferoxamine and cobalt chloride and can be used to probe the hypoxia signaling pathway. This cell line can be adapted for high-throughput screening of agonist or antagonist compound libraries. Candidate drugs can also be tested for dose response against this cell line. Academic and non-profit customers, please inquire for special pricing.

TRUSTED_SUSTAINABILITY

Specifications

Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Product Line CellSensor
Quantity 1 Vial
Target Entry HIF1 alpha, Hypoxia pathway
System Type CellSensor™
Druggable Target Signaling Pathway

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.