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Thermo Scientific™ CellSensor™ NFκB-bla RA 1 Cell Line
Description
The CellSensor™ NFκB-bla RA-1 cell line contains a beta-lactamase reporter gene under control of the NFκB response element stably integrated into Ramos 1 (RA-1) cells. To construct this cell line, the NFκB -bla construct was transduced into RA-1 cells by lentivirus. Flow cytometry was used to isolate cells responsive to Tumor Necrosis Factor (TNFThis cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading time, as well as Z' and EC50 concentrations for TNF. The CellSensor™ NFκB-bla RA-1 cell line is responsive to TNFand can be used to probe NFκB signaling pathways, which are involved in the regulation of apoptosis, viral replication, tumorigenesis, inflammation, and various autoimmune diseases. This cell line can be adapted for high-throughput screening for agonists or antagonists with compound libraries. Candidate drugs can be tested for dose response against this cell line. Academic and non-profit customers, please inquire for special pricing.
Specifications
Specifications
| Assay Entry | Cell-based beta lactamase reporter gene |
| Cell State | Dividing Cells |
| Content And Storage | CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use. |
| Product Line | CellSensor™ |
| Quantity | 1 Vial |
| Target Entry | TNF alpha, NIK, TLR, I kappa B, IRAK, IKK, NF-kappa B pathway |
| System Type | CellSensor™ |
| Druggable Target | Signaling Pathway |
For Research Use Only. Not for use in diagnostic procedures.
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