Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. General Cell Based Assays
  6. K1538

Thermo Scientific™ CellSensor™ NFAT bla HEK 293T Cell Line

Catalog No. K1538
Encompass_Preferred
Change view
Click to view available options
Quantity:
1 Vial
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
K1538 1 Vial
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. K1538 Supplier Thermo Scientific™ Supplier No. K1538
Only null left
Add to Cart
Add to Cart
Request a Quote

Description

The CellSensor™ NFAT-bla HEK 293T cell line contains a beta-lactamase reporter gene under the control of the Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into HEK 293T cells.

The CellSensor™ NFAT-bla HEK 293T cell line contains a beta-lactamase reporter gene under the control of the Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into HEK 293T cells. Fluorescence activated cell sorting (FACS) was used to isolate clones responsive to stimulation of the NFAT pathway by PMA (Phorbol 12-Myristate 13-Acetate) and Thapsagargin (Thaps). The cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading conditions, and EC50 concentration of Thaps at fixed concentration of PMA. The CellSensor™ NFAT-bla HEK 293T cell line responds to agonist treatment as expected from the literature and can be adapted for high-throughput screening (including coupling of receptors to the NFAT pathway) for agonists or antagonists with compound libraries. Candidate drugs can also be tested for dose response against this cell line. Academic and non-profit customers, please inquire for special pricing.

TRUSTED_SUSTAINABILITY

Specifications

Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Product Line CellSensor
Quantity 1 Vial
Target Entry PKC, PKC and calcium pathway
System Type CellSensor™
Druggable Target Signaling Pathway

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.