Invitrogen™ CEP135 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA536440

Catalog No. PIPA536440

Description

This antibody detects endogenous protein at a molecular weight of 135 kDa. Purity is >95% by SDS-PAGE.

LATS1 is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.

Specifications

• Antigen: CEP135
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CEP135
• Gene Alias: BC062951; centrosomal protein 135; centrosomal protein 135kDa; centrosomal protein 4; Centrosomal protein of 135 kDa; centrosomal protein of 135 kDa; LOW QUALITY PROTEIN: centrosomal protein of 135 kDa; centrosome protein cep135; Cep135; Cep4; Gm1044; KIAA0635; MCPH8; RGD1310726
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 305288, 9662
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q66GS9
• Gene Symbols: CEP135
• Immunogen: Synthetic peptide corresponding to amino acids 1087-1140 of human CEP135.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Rat
• Product Type: Antibody
• Isotype: IgG