MilliporeSigma™ ChIC/Cut & Run Kit (pA-MN/pG-MN)

Description

ChIC/Cut & Run Kit (pA-MN) low signal-to-noise ratio and less starting sample compared to ChIP

• Benefits:
  • Extremely low signal-to-noise ratio than cross-linking ChIP-seq
  • Cross-linking eliminated (no sonicators or spin steps needed)
  • Lower number of starting cells (minimum 100 cell for histone modification and 1,000 cells for a transcription factor)
  • Shortened procedure time- from cells to purified DNA completed within one day (min. 3 hours)
  • No special equipment needed
• Includes:
  • Concanavalin A-coated magnetic beads
  • Binding Buffer
  • Wash Buffer
  • Spermidine
  • 0.5M EDTA
  • Rinse Buffer
  • Incubation buffer
  • Stop Solution
  • 2% Digitonin containing DMSO
  • RNase A
  • Spike-in DNA
  • Precipitate Enhancer
  • Proteinase K Solution
  • Protease Inhibitor Cocktail III, Animal Free **Contains DMSO
  • Rabbit IgG Purified
  • Anti-H3K27me3 rabbit monoclonal antibody
  • Protein A- micrococcal nuclease and protein G-micrococcal nuclease fusion proteins blend (pA-MN/pG-MN) 

Specifications

• Content And Storage: Store ChIC box 1 (at 2°C to 8°C and ChIC box 2 (at -20 °C))
• For Use With (Application): Low Signal-to-Noise Ratio, Less Starting Sample
• Quantity: 24 Reactions

For research purposes only