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Life Diagnostics Inc Chicken Ex-FABP ELISA kit
SDP

Supplier:  Life Diagnostics Inc EXFABP5

Encompass_Preferred

The assay uses two chicken Ex-FABP antibodies developed at Life Diagnostics; one for solid-phase immobilization (microtiter wells) and one, conjugated to horseradish peroxidase (HRP), for detection. Standards and diluted samples are incubated in the microtiter wells with HRP-conjugate for one hour. This results in Ex-FABP molecules being sandwiched between the immobilization and detection antibodies. The wells are washed and TMB is added and incubated for 20 minutes. If Ex-FABP is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of Ex-FABP is proportional to absorbance and is derived from a standard curve.

Catalog No. NC1793544


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