Invitrogen™ Rat CINC-1 (CXCL1) ELISA Kit

ELISA

Supplier:  Invitrogen™ ERCXCL1X5

Catalog No. 50-254-5379

Description

The Rat CINC-1 (CXCL1) ELISA quantitates Rt CINC-1 in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt CINC-1. Principle of the method The Rat CINC-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

Specifications

• Accession Number: P14095
• Assay Sensitivity: 50 pg/ml
• Description: CINC-1 (CXCL1) ELISA Kit, 96 Assays, 1 Kit, Rat Species, Chemokines and Receptors Protein Family, Immunology Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of CINC-1 (CXCL1) in Samples, Using a Microplat
• Assay: Solid-phase sandwich ELISA
• Detection Method: Colorimetric
• Protein Family: Chemokine & Receptors
• Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, IL-1 alpha, IL-1 beta, IL-6, IL- 10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF.
• Sufficient For: 96 assays
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CINC-1,Gro1
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 5 x 96 Tests
• Research Discipline: Immunology
• Storage Requirements: 2°C to 8°C
• Target: CXCL1 (GRO alpha)
• Total Assay Time: 4 hrs 45 mins

• Assay Range: 234.4 to 15,000 pg/mL
• Calibration Range: 300 pg/mL to 15000 pg/mL
• Detectable Analytes: CINC-1 (CXCL1)
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Substrate Type: TMB
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 81503
• Host Species: Rat
• Intraassay CV: <10
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Synonym: CINC-1, Gro1
• Test Time: 1 hrs 20 mins