Invitrogen™ Rat Clusterin (CLU) ELISA Kit

ELISA

Supplier:  Invitrogen™ ERCLUX5

Catalog No. 50-254-5396

Description

The Rat Clusterin (Rt CLU) ELISA quantitates Rt Clusterin in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt Clusterin. Principle of the method The Rat Clusterin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.

Specifications

• Accession Number: P05371
• Assay Sensitivity: 1.3 ng/mL
• Description: Clusterin (CLU) ELISA Kit, 96 Assays, 1 Kit, Rat Species, Histones Protein Family, Oncology Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Clusterin (Clu) in Samples, Using a Microplate Reader
• Assay: Solid-phase sandwich ELISA
• Detection Method: Colorimetric
• Protein Family: Histones
• Specificity: This ELISA pair antibody detects rat and mouse Clusterin. Other species not determined.
• Sufficient For: 96 assays
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Clu
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 5 x 96 Tests
• Research Discipline: Oncology
• Storage Requirements: 2°C to 8°C
• Target: Apolipoprotein J
• Total Assay Time: 4 hrs 45 mins

• Assay Range: 1.311 to 320 ng/mL
• Calibration Range: 1.3 ng/ml to 320 ng/ml
• Detectable Analytes: Clusterin (CLU)
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Substrate Type: TMB
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 24854
• Host Species: Rat
• Intraassay CV: <10
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
• Synonym: APOJ, CLI, DAG, RATTRPM2B, SGP-2, SGP2, SP-40, SP40, TRPM-2, TRPM2B, Trpm2, Trpmb
• Test Time: 1 hrs 20 mins