Novus Biologicals™ Coagulation Factor III/Tissue Factor Antibody Pair [HRP]

Catalog No.	Quantity
NB010339	15 Plates

Catalog No. NB010339 Supplier Novus Biologicals™ Supplier No. NBP27965015PLATES

Description

Polyclonal/Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Rat Coagulation Factor III/Tissue Factor.

Specifications

• Assay Range: 23.45 pg/mL
• Assay Sensitivity: 23.45-1500 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Rat Coagulation Factor III/Tissue Factor.
• Target Species: Rat
• For Use With (Application): Sandwich ELISA
• Gene Alias: CD142, CD142 antigen, Coagulation factor III, coagulation factor III (thromboplastin, tissue factor), FLJ17960, TF, TFA, Thromboplastin, tissue factor
• Gene ID (Entrez): 2152
• Gene Symbol: F3
• Host Species: Rabbit/Mouse
• Kit Contents:
  Standard: Each vial contains 80 ng of recombinant Rat Coagulation Factor III/Tissue Factor.
  Capture Antibody: 0.5 mg/mL of mouse anti-Rat Coagulation Factor III/Tissue Factor monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of rabbit anti-Coagulation Factor III/Tissue Factor polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cancer
• Storage Requirements: Storage is content dependent.
• Target: Coagulation Factor III/Tissue Factor
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1500 pg/mL is recommended.