Invitrogen™ Collagen IV Monoclonal Antibody (1042), eFluor™ 660, eBioscience™

Catalog No.	Quantity
50-255-0787	100 μg

Catalog No. 50-255-0787 Supplier Invitrogen™ Supplier No. 50987182

Description

Mouse Monoclonal Antibody

Description: This monoclonal antibody 1042 recognizes human collagen Type IV. Collagens are the main components of the extracellular matrix and provide the tensile strength to tissues. There are four main types of collagen (I, II, III and IV) which contribute to the connective tissue. Expression of type IV is restricted to the basal lamina. It contains two alpha-1 and one alpha-2-like chains. Collagen Type IV is associated with disorders such as Alport syndrome and Goodpasture's syndrome. Applications Reported: This 1042 antibody has been reported for use in immunohistochemical staining, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This 1042 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

Collagen IV is a type of collagen protein that is a major component of the basement membrane in various tissues, including the kidneys, lungs, and skin. It forms a network-like structure that provides support and stability to the surrounding tissue. Collagen IV is composed of three alpha chains, which are encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes can lead to various genetic disorders, such as Alport syndrome and Goodpasture syndrome, which affect the kidneys and lungs, respectively.

Specifications

• Antigen: Collagen IV
• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunocytochemistry
• Classification: Monoclonal
• Clone: 1042
• Concentration: 0.2 mg/mL
• Conjugate: eFluor 660
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: COL4A2
• Gene Accession No.: P02462, P08572, P29400, P53420, Q01955, Q14031
• Gene Alias: [a]3(IV); [a]4(IV); alpha 1 type IV collagen; alpha type IV collagen basement membrane; alpha1(IV) collagen; alpha-2 collagen type IV; alpha-3 type IV collagen; alpha3(IV); Arresten; Bru; BSVD; CA44; Canstatin; Col4a1; Col4a-1; COL4A1 NC1 domain; Col4a2; Col4a-2; COL4A3; col4a4; Collagen 4; Collagen alpha-1(IV) chain; collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; collagen alpha-4(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen type IV alpha 1 chain; collagen type IV alpha 2; collagen type IV alpha 2 chain; collagen type IV alpha 3 chain; collagen type IV alpha 4 chain; collagen type IV alpha3 chain; collagen, type IV, alpha 1; collagen, type IV, alpha 2; collagen, type IV, alpha 3; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; Del(8)44H; Del(8)Bru44H; E130010M05Rik; Goodpasture antigen; ICH; LOW QUALITY PROTEIN: collagen alpha-1(IV) chain; LOW QUALITY PROTEIN: collagen alpha-3(IV) chain; LOW QUALITY PROTEIN: collagen alpha-4(IV) chain; POREN2; procollagen alpha1(IV); procollagen, type IV, alpha 1; procollagen, type IV, alpha 2; procollagen, type IV, alpha 3; procollagen, type IV, alpha 4; RATOR; Raw; retinal anterior wiring; Svc; tumstatin; Type IV Collagen
• Gene Symbols: COL4A1, COL4A2, Col4a3, COL4A4, COL4A5, COL4A6
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1282, 1284, 1285, 1286, 1287, 1288
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b κ

Frequently Asked Questions (FAQs)

I am unable to analyze my cells stained with eFluor Organic Dyes today. What options do I have?

Our options will depend on the samples you are analyzing.

If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.

For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.

Can the eFluor Organic fluorochromes be used for intracellular staining?

Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).

Is the eFluor 660 fluorochrome compatible with Anti-Cy5/Alexa Fluor 647 beads?

Yes, in-house studies have demonstrated that the eFluor 660 fluorochrome is recognized by Anti-Cy5/Alexa Fluor 647 beads. Side by side studies with Alexa Fluor 647 versus eFluor 660 conjugated antibodies have demonstrated comparable results.

What buffers are compatible with the eFluor Organic fluorochromes and eVolve QDots?

The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.

Are the eFluor Organic Dyes photo-labile?

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.