The collagenase product is a mixture of enzymes secreted by C. histolyticum, with different products differentiated by the relative ratios of the 10-18 components found in the secreted enzymes. The main components are two collagenases, clostripain, and a neutral protease. The synergistic action of these enzymes degrade collagen and other intracellular material. The action of both collagenase enzymes and the neutral protease is necessary for effective release of cells from tissue. Various types of collagen are the natural substrates for collagenase.
Unit Definition: One unit will liberate peptides from collagen equivalent in ninhydrin color to 1.0µmol of L-leucine in 5 hr. at pH 7.4 and 37°C in presence of calcium ions. Contains clostripain, neutral protease and trypsin activities.
|Store at 4°C. Desiccate.|
|Preparation of isolated cell suspensions|
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