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Description
Specifications
Specifications
| For Use With (Application) | Enzyme Activity |
| Name | Collagenase Type 1 (C. histolyticum) |
| Preparation Method | Native protein purified from Clostridium histolyticum |
| Quantity | 100 mg |
| Storage Requirements | Store at 4°C on dry atmosphere. For primary cell isolation, culture and tissue dissociation: Reconstituted in balanced salt solutions (such as Hank's, Earle's and others) at concentrations from 0.05% to 0.5% (w/v). For enzymetic assay: Reconstituted in 0.05 M TES buffer with 0.36 mM calcium chloride, pH 7.5 at a concentration of 1.0 mg/ml. |
| Regulatory Status | RUO |
| Common Name | COLLAGENASE |
| Assays | Collagenase Assay; Epithelial, liver, lung and adrenal primary cell isolations. |
| Biological Activity | ≥125 units per mg dry weight. One Unit releases one micromole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5. |
| Species | Clostridium species |
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