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R&D Systems™ CometAssay Software

Catalog No. 4260000CS
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Description: Simplifies assay performance from end-to-end, and assures sensitive, consistent quantitative results
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Catalog No.
42-600-00CS Description: Simplifies assay performance from end-to-end, and assures sensitive, consistent quantitative results
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Description

Required for using CometChip™ Electrophoresis Starter Kit

  • Quantitative Analysis using Comet™ Analysis Software
    • Image capture
      • Auto set the camera on untreated cells to avoid saturation of undamaged heads
      • Position comet head to the left and comet tail to the right
      • Take all images at same magnification (4X to 10X) and camera settings
      • Save images as TIFF, JPEG or PNG
    • Edit well images to remove comets not located within pattern of nonoverlapping micropores
    • Determine median % DNA in the Tail for each of the 96 macrowells
    • Untreated controls serve as a baseline for comparison to treated cells. Generally, <10% DNA in the tail is indicative of a healthy population
    • Use boxplots to summarize data graphically

Specifications

Description Simplifies assay performance from end-to-end, and assures sensitive, consistent quantitative results
For Use With (Equipment) CometAssay™ System and high throughput CometChip™ technology, along with Microsoft Windows™ Operating System (Windows 10, Windows 8/8.1, Windows 7)
For Use With (Application) Rapidly evaluate large numbers of cells, related to each treatment group or screening target in a study, and generate summary statistics based on the corresponding numeric results
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