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Gibco™ Control Vesicles

Catalog No. GM0003
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GM0003 0.5 mL
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Description

Control Vesicles

These Control Vesicles are insect-derived purified plasma membranes with no inserted transport proteins for use as a negative control in vesicular transport assays. Our Control Vesicles control for nonspecific absorption and transport of test substrates to insect plasma membranes.

Control Vesicle Details
Control Vesicles are a negative control product for use with ABC transporter vesicles. They are prepared from purified plasma membranes isolated from an insect cell system (Sf9 cells). Due to the nonspecific transport activity and absorption properties of test substrates to Sf9 cell membranes use of these Control Vesicles is highly recommended.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
TRUSTED_SUSTAINABILITY

Specifications

Host Species S. frugiperda, Spodoptera frugiperda
Grade Research Grade
For Use With (Application) Pharma and Biopharma
Packaging Vial
Content And Storage Store Control Vesicles at -80°C.
Shipping Condition Dry Ice
Source S. frugiperda,Spodoptera frugiperda
Quantity 0.5 mL
Product Type Control Vesicles
Form Frozen

Frequently Asked Questions (FAQs)

What is the supplied quantity of ATP-binding cassette (ABC) Transporter Vesicles?

The quantity supplied is 2.5 mg total protein/500 µL per vial. The protein concentration is 5 mg/mL. When our recommended protocol is followed, 50 vesicular transport assays or 125 ATPase assays can be performed with one vial of ABC Transporter Vesicles product.
What is the storage condition for ATP-binding cassette (ABC) Transporter Vesicles?

We recommend storing them at -80 degrees C.
What are ATP-binding cassette (ABC) Transporter Vesicles?

Prepared from Sf9 cells which have been engineered to over-express specific ABC transporters, ATP-binding cassette (ABC) Transporter Vesicles are “inside-out” vesicles designed for use in vesicular transport assays. They allow the investigation of possible interactions between specific ABC transporters and test compounds. ABC Transporter Vesicles provide high levels of transporter activity with low background, giving a clear signal if the test compound is a substrate or inhibitor of a specific efflux transporter.

While ABC transporters typically mediate the efflux of substrates from cells, transporters expressed on these inside-out vesicles import substrates into the vesicles. It is therefore possible to quantitatively evaluate transport activity for the test compound by determining the amount incorporated into the vesicles.
Note: One vial of vesicle product can perform 50 vesicular transport assays.

For Research Use Only. Not for use in diagnostic procedures.

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