Coomassie Brilliant Blue G-250, MP Biomedicals™

Description

Useful dye for SDS gels as it readily stains proteins with minimal background color

• It is a protein stain in electrophoresis.
• It has been used in the Bradford dye-binding protein assay.
• A mechanism for dye binding to protein has been proposed, based on measuring Coomassie Brilliant Blue G-250 (CBBG) absorbance spectra during titration of the dye reagent in the absence of protein and its response to different polyamino acids.
• Brilliant Blue G has been used for determining critical micelle concentration of detergents.

Specifications

Chemical Identifiers

• CAS: 6104-58-1
• Molecular Formula: C47H48N3NaO7S2
• MDL Number: MFCD00078482

• Beilstein: 5230822
• Synonym: Acid Blue 90, Coomassieg250, CBBG
• Solubility Information: Soluble in hot water (50mg/mL), ethanol (40mg/mL), methanol and 10% trichloroacetic acid. Soluble in water (1mg/mL) yielding dark Blue Solution.
• InChI Key: CMGWKYXJIYYESN-UHFFFAOYSA-M
• Molecular Weight (g/mol): 854.02
• Loss on Drying: 0.045
• UV_Vis: λ max (methanol) 608 ± 3 nm
• Color: Dark Reddish Purple
• Quantity: 100 g
• Physical Form: Powder

Safety and Handling

storageNote1

Store at Room Temperature (15-30°C).

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