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MilliporeSigma™ CpGenom™ 5-hmC Quantitation Kit

Catalog No. 1710091 Shop All MilliporeSigma Products
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96-well Plate
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Catalog No. Quantity
17-100-91 96-well Plate
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Catalog No. 17-100-91 Supplier MilliporeSigma™ Supplier No. 1710091

Description

Includes

  • Plate Coating Buffer (15 mL)
  • 10X Assay Buffer (30 mL)
  • HRP Detection Reagent (15 mL)
  • 96-well Assay Plate and 8-well Strip Caps (1 plate/12 strips)
  • 5-hmC Antibody (1mg/mL)
  • 100x HRP Detection Antibody (1 mg/mL)
  • 5-hmC DNA Quantitation Standards - 5 vials for creation of standard curve (100 ng/μL each)

Requires

Incubator, thermal cyclers, Plate reader capable of 405 nm detection, Multichannel pipettor (recommended), Parafilm™, Pipette tips, dilution tubes, and other assorted consumables

Ideal for experiments where global and/or tissue-specific detection and quantitation of 5-hmC DNA is required with quantitation based on set of precisely calibrated standards

  • Compatible with genomic DNA from cultured cells or tissues
  • Includes set of 5-hmC of standards for precise quantitation
  • Streamlined, simple, automation-friendly ELISA-based protocol
  • Sensitive colorimetric detection with a limit of quantitation down to 0.03% of input DNA.
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store components at the temperatures indicated on the labels
For Use With (Application) Determination of relative/absolute levels of 5-hydroxymethyl cytosine (5-hmC) in genomic DNA samples
Quantity 96-well Plate
Stability Kit components are stable for 6 months
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