MilliporeSigma™ CpGenom™ 5-hmC Quantitation Kit

Description

Includes

• Plate Coating Buffer (15 mL)
• 10X Assay Buffer (30 mL)
• HRP Detection Reagent (15 mL)
• 96-well Assay Plate and 8-well Strip Caps (1 plate/12 strips)
• 5-hmC Antibody (1mg/mL)
• 100x HRP Detection Antibody (1 mg/mL)
• 5-hmC DNA Quantitation Standards - 5 vials for creation of standard curve (100 ng/μL each)

Requires

Incubator, thermal cyclers, Plate reader capable of 405 nm detection, Multichannel pipettor (recommended), Parafilm™, Pipette tips, dilution tubes, and other assorted consumables

Ideal for experiments where global and/or tissue-specific detection and quantitation of 5-hmC DNA is required with quantitation based on set of precisely calibrated standards

• Compatible with genomic DNA from cultured cells or tissues
• Includes set of 5-hmC of standards for precise quantitation
• Streamlined, simple, automation-friendly ELISA-based protocol
• Sensitive colorimetric detection with a limit of quantitation down to 0.03% of input DNA.

Specifications

• Content And Storage: Store components at the temperatures indicated on the labels
• For Use With (Application): Determination of relative/absolute levels of 5-hydroxymethyl cytosine (5-hmC) in genomic DNA samples
• Quantity: 96-well Plate
• Stability: Kit components are stable for 6 months