MilliporeSigma™ CpGenome™ Direct Prep Bisulfite Modification Kit

Description

Includes

• 2X Extraction Buffer
• Modification Reagent
• Conversion Buffer
• Resuspension Buffer
• Equilibration Buffer
• Binding Buffer
• Wash Buffer I
• Wash Buffer II
• Elution Buffer
• Proteinase K
• Proteinase K Storage Buffer
• Desalting Columns
• Collection Tubes

Requires

MILLIPLEX MAP Cell Signaling Buffer and Detection Kit

Simple and reliable bisulfite conversion of DNA directly from variety of starting materials, including cultured cells, blood, fresh tissue and fixed tissue samples

• Bisulfite conversion directly from cells, tissues, blood and FFPE samples without DNA purification
• Sensitive bisulfite-conversion from as few as 10 cells or as low as 50pg of input DNA
• Fast and simple, streamlined protocol for one-step bisulfite conversion
• In-column desulfonation allows recovery of DNA without additional precipitation steps for more consistent results
• Suitable for downstream analysis by methlylation specific PCR, restriction digestion, sequencing, microarray hybridization, etc.

Specifications

• For Use With (Application): Bisulfite conversion directly from a variety of starting materials, including cultured cells, blood, fresh tissue & fixed tissue samples
• Quantity: 50 Reactions