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Sigma Aldrich Fine Chemicals Biosciences CRISPR Lenti Non Targeting1EA
Supplier: Sigma Aldrich Fine Chemicals Biosciences CRISPR12V1EA
The Sigma lenti CRISPR Non-Targeting Control is a lentiviral system which includes a gRNA sequence that does not target known human mouse and rat genes. The non-targeting control lentivirus is useful as a negative control in experiments using Sigma CRISPR lentiviral clones. The Sigma lenti CRISPR non-targeting control uses a one-plasmid system consisting of a Ef1-alpha-driven Cas9 a U6-driven guide RNA (non-targeting) with both Puromycin and GfP co-expressed with Cas9. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. Also self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP001).
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