Invitrogen™ CRM1 Monoclonal Antibody (5G3)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA527879

Catalog No. PIMA527879

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: rat liver tissue, rat lung tissue, mouse liver tissue, mouse lung tissue, human HepG2 whole cell, human SMMC-7721 whole cell, human Hela whole cell, human Jurkat whole cell. IHC: human lung cancer tissue, human intestinal cancer tissue. ICC/IF: U20S cell. Flow: SiHa cell.

Exportin - 1 (XPO1) mediates the nuclear export of cellular proteins bearing a leucine-rich nuclear export signal (NES) and RNAs. In the nucleus, in association with RANBP3, XPO1 binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex is mediated through binding to nucleoproteins. Upon transit of a nuclear complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP cause release of the cargo from the export receptor. The directionality of the nuclear export is thought to be conferred by an asymmetric distribution of the GTP and GDP bound forms of Ran between the cytoplasm and nucleus.

Specifications

• Antigen: CRM1
• Classification: Monoclonal
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and 0.05mg sodium azide
• Gene Accession No.: O14980, Q6P5F9, Q80U96
• Gene Symbols: XPO1
• Immunogen: E.coli-derived human CRM1 recombinant protein (Position: N966-D1071).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 5G3
• Conjugate: Unconjugated
• Gene: XPO1
• Gene Alias: AA420417; chromosome region maintenance 1 homolog; chromosome region maintenance 1 protein homolog; CRM1; emb; Exp1; exportin 1; exportin 1 (CRM1 homolog, yeast); exportin 1 (CRM1, yeast, homolog); exportin 1, CRM1 homolog; exportin 1, CRM1 homolog (yeast); exportin-1; exportin-1 (required for chromosome region maintenance); Xpo1
• Host Species: Mouse
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 103573, 7514, 85252
• Content And Storage: -20°C
• Form: Lyophilized