Invitrogen™ CRM1 Monoclonal Antibody (5G3)

Catalog No.	Quantity
PIMA527879	100 μg

Catalog No. PIMA527879 Supplier Invitrogen™ Supplier No. MA527879

Description

Mouse Monoclonal Antibody

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: rat liver tissue, rat lung tissue, mouse liver tissue, mouse lung tissue, human HepG2 whole cell, human SMMC-7721 whole cell, human Hela whole cell, human Jurkat whole cell. IHC: human lung cancer tissue, human intestinal cancer tissue. ICC/IF: U20S cell. Flow: SiHa cell.

Exportin - 1 (XPO1) mediates the nuclear export of cellular proteins bearing a leucine-rich nuclear export signal (NES) and RNAs. In the nucleus, in association with RANBP3, XPO1 binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex is mediated through binding to nucleoproteins. Upon transit of a nuclear complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP cause release of the cargo from the export receptor. The directionality of the nuclear export is thought to be conferred by an asymmetric distribution of the GTP and GDP bound forms of Ran between the cytoplasm and nucleus.

Specifications

• Antigen: CRM1
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 5G3
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 4mg trehalose and 0.05mg sodium azide
• Gene: XPO1
• Gene Accession No.: O14980, Q6P5F9, Q80U96
• Gene Alias: AA420417; chromosome region maintenance 1 homolog; chromosome region maintenance 1 protein homolog; CRM1; emb; Exp1; exportin 1; exportin 1 (CRM1 homolog, yeast); exportin 1 (CRM1, yeast, homolog); exportin 1, CRM1 homolog; exportin 1, CRM1 homolog (yeast); exportin-1; exportin-1 (required for chromosome region maintenance); Xpo1
• Gene Symbols: XPO1
• Host Species: Mouse
• Immunogen: E.coli-derived human CRM1 recombinant protein (Position: N966-D1071).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 103573, 7514, 85252
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG2b