Invitrogen™ CRY1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1527

Catalog No. PA1527

Description

PA1-527 detects recombinant human cryptochrome 1 (Cry1). PA1-527 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~70 kDa protein representing full length recombinant human Cry1 protein. PA1-527 immunizing peptide corresponds to amino acid residues 594-606 from mouse Cry1. PA1-527 immunizing peptide (Cat. # PEP-103) is available for use in neutralization and control procedures.

Various biochemical, physiological and behavioral processes display circadian rhythms controlled by an internal biological clock. The central gears driving this clock appear to be composed of an autoregulatory transcription/post translation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

Specifications

• Antigen: CRY1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CRY1
• Gene Alias: AU020726; AU021000; Cry1; cryptochrome 1 (photolyase-like); cryptochrome circadian clock 1; cryptochrome-1; PHLL1
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12952, 1407
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P97784, Q16526
• Gene Symbols: CRY1
• Immunogen: Synthetic Peptide: C Q(594) S V G P K V Q R Q S S N(606).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG