CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, eBioscience™, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen 14799281

Catalog No. 50-137-26

Description

Description: The polyclonal antibody reacts with both mouse and rat SDF-1 alpha. It was was generated using E. coli-expressed N terminal amio acids 20-89 of mouse SDF-1 alpha as an immunogen. SDF-1 alpha (stromal cell-derived factor-1 alpha), also known as pre-B-cell growth-stimulating factor (PBSF), is a 70-amino acid CXC chemokine originally cloned from a bone marrow stromal cell line. Targeted deletion of the SDF-1 gene resulted in defects of B-cell lymphopoiesis and bone marrow myelopoiesis. SDF-1 has been shown to be chemotactic for lymphocytes. In addition, SDF-1 was recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV-1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV-1 entry. Applications Reported: This polyclonal has been reported for use in immunoblotting (WB), immunoprecipitation, and immunohistochemistry. Cross-reactivity to SDF-1 alpha of other species has not been determined. Applications Tested: To achieve best results, titrate antibody by dilution for each application as follows: Western Blot 1:1000 Immunoprecipitation 1:300-500 Immunohistochemistry 1:100-500. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: CXCL12 alpha (SDF-1 alpha)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CXCL12
• Gene Alias: 12-O-tetradecanoylphorbol 13-acetate repressed protein 1; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 12; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); CXC; CXC motif chemokine; C-X-C motif chemokine 12; C-X-C motif chemokine ligand 12; CXCL; Cxcl12; H-SDF-1-alpha; Intercrine reduced in hepatomas; IRH; Pbsf; pre-B cell growth-stimulating factor; pre-B-cell growth-stimulating factor; SCYB12; SDF 1 a; SDF 1 alpha; SDF 1a; SDF1; SDF-1; SDF1 alpha; SDF-1 gamma; SDF1A; SDF-1-alpha(3-67); SDF1B; SDF-1-beta(3-72); SDF1a; Stromal cell-derived factor 1; stromal cell-derived factor-1 alpha; stromal cell-derived factor-1 gamma; thymic lymphoma cell-stimulating factor; TLSF; Tpar1
• Host Species: Rabbit
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P40224
• Gene Symbols: CXCL12
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20315, 24772
• Content And Storage: 4° C
• Form: Liquid

For Research Use Only.