CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
500P31550UG	Pepro Tech Inc 500P31550UG	50 μg
500P315100	Pepro Tech Inc 500P315100UG	100 μg

Description

AA Sequence of recombinant protein: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat SDF-1alpha (CXCL12). Anti-Rat SDF-1alpha (CXCL12)-specific antibody was purified by affinity chromatography employing an immobilized Rat SDF-1alpha (CXCL12) matrix. Sandwich ELISA: To detect Rat SDF-1alpha (CXCL12) by sandwich ELISA (using 100 μL/well antibody solution), a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat SDF-1alpha (CXCL12) (500-P315Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat SDF-1alpha (CXCL12). Western Blot: To detect Rat SDF-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat SDF-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: CXCL12 alpha (SDF-1 alpha)
• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Rat
• Gene Alias: 12-O-tetradecanoylphorbol 13-acetate repressed protein 1; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 12; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); CXC; CXC motif chemokine; C-X-C motif chemokine 12; C-X-C motif chemokine ligand 12; CXCL; Cxcl12; H-SDF-1-alpha; Intercrine reduced in hepatomas; IRH; Pbsf; pre-B cell growth-stimulating factor; pre-B-cell growth-stimulating factor; SCYB12; SDF 1 a; SDF 1 alpha; SDF 1a; SDF1; SDF-1; SDF1 alpha; SDF-1 gamma; SDF1A; SDF-1-alpha(3-67); SDF1B; SDF-1-beta(3-72); SDF1a; Stromal cell-derived factor 1; stromal cell-derived factor-1 alpha; stromal cell-derived factor-1 gamma; thymic lymphoma cell-stimulating factor; TLSF; Tpar1
• Gene Symbols: CXCL12
• Primary or Secondary: Primary
• Content And Storage: -20°C

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene ID (Entrez): 24772
• Immunogen: E.coli-derived Recombinant Rat SDF-1alpha (CXCL12).
• Purification Method: Antigen Affinity Chromatography
• Gene: CXCL12