Invitrogen™ Cy3™ Dye-Labeled Anti-miR™ Negative Control #1

Catalog No.	Quantity
AM17011	5 nmol

Catalog No. AM17011 Supplier Invitrogen™ Supplier No. AM17011

Description

Cy3™ Dye-Labeled Anti-miR™ Negative Control #1

Invitrogen™ Cy™3 dye–labeled synthetic miRNA inhibitor for miRNA functional analysis. 5 nmol is supplied, purified and ready to transfect. Invitrogen™ Anti-miR™ miRNA Inhibitors are chemically modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous microRNA (miRNA) molecules. These ready-to-use inhibitors can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. Cy™3 Dye Labeled Anti-miR™ Negative Control #1 is a random sequence Anti-miR molecule that has been extensively tested in human cell lines and tissues and validated to produce no identifiable effects on known miRNA function. It is used for monitoring transfection efficiency in transfection experiments using Anti-miR miRNA Inhibitors. The fluorescent label enables direct observation of cellular uptake, distribution, and localization of the control. The molecule is labeled at its 5' end and has the same oligonucleotide sequence as unlabeled Anti-miR™ miRNA Inhibitor Negative Control #1 (SKU #AM17010).

Accessory Products:
Also available are Anti-miR™ miRNA Inhibitors (SKU #AM17000 and #AM17001). To select your miRNA sequence and order, please visit the GeneAssist™ miRNA Workflow Builder. FAM™ dye-labeled Anti-miR™ Negative Controls (SKU #AM17012) is available. The Pre-miR™ miRNA Starter Kit (SKU #AM1540) can be used to optimize transfection of Pre-miR™ miRNA Precursors.

Specifications

• Content And Storage: Anti-miR™ miRNA is provided dried down in a single tube, along with Nuclease-free Water for resuspension, and should be stored at –20°C. It is guaranteed for 1 year when stored dried at –20°C.
• Control Type: Negative Control
• RNAi Type: miRNA Inhibitor
• Species: Human, Mouse, Rat
• For Use With (Application): miRNA & Non-Coding RNA Analysis
• Label or Dye: Cy3
• Product Line: Ambion™, Anti-miR™, Cy™3
• Product Type: miRNA Inhibitor
• Quantity: 5 nmol
• Shipping Condition: Dry Ice

Frequently Asked Questions (FAQs)

Where can I find more information about published miRNA sequences and annotation?

Please visit miRBase: The microRNA database (http://www.mirbase.org/).

I would like to order a mimic or inhibitor with a 5' label. Can you do this?

We can label our Pre-miR and Anti-miR miRNA, but do not offer labeled mirVana Mimics and inhibitors. 5' modifications include Alexa Fluor 555, Alexa Fluor 647, FAM, and Cy3. Request a quote here (http://www.thermofisher.com/us/en/home/global/forms/custom-sirna-mirna-quote-request.html).

The miRNA sequence I need to target is not in the miRBase database. Can I order a custom mimic or inhibitor?

Yes, submit your unpublished miRNA sequences and/or order custom modified miRNA here (http://www.thermofisher.com/order/custom-genomic-products/tools/mirna/).

What is the difference between Invitrogen Anti-miR inhibitors & Invitrogen Pre-miR miRNA Precursors and mirVana miRNA mimics and inhibitors?

Invitrogen Anti-miR inhibitors & Invitrogen Pre-miR miRNA Precursors are our first generation mimic and inhibitors. Invitrogen Anti-miR miRNA Inhibitors are chemically modified, single stranded nucleic acids designed to specifically bind and inhibit endogenous microRNA (miRNA) molecules. These are ready to use inhibitors that can be introduced into cells using transfection or electroporation. Pre-designed inhibitors have been created for all miRNAs listed in miRBase version 15.0.
Invitrogen Pre-miR miRNA Precursors are small, chemically modified double-stranded RNA molecules designed to mimic endogenous mature miRNAs. These ready-to-use miRNA mimics, which are similar to, but not identical to siRNAs, can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. Please note, Pre-miR miRNA Precursors are not hairpin constructs and should not be confused with pre-miRNAs.
mirVana miRNA mimics and inhibitors are our second generation of mimics and inhibitors. mirVana miRNA mimics are small, chemically double-stranded RNAs that mimic endogenous mature miRNAs and enable miRNA functional analysis by up-regulation of miRNA activity. They have enhanced specificity over the first generation mimics, are delivered as HPLC purified, and ready to transfect.
miRNA inhibitors are small, chemically modified single-stranded RNA molecules designed to specifically bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity. They have enhanced potency over our first generation inhibitors. Pre-designed inhibitors were created for all miRNAs listed in miRBase version 19.0. They come delivered as HPLC purified, and ready to transfect. Learn more about mirVana miRNA mimics and inhibitors through this video (https://www.youtube.com/watch?feature=player_embedded&v=Q3MO1lmT0kw). Both mimics and inhibitors are available individually or as libraries.
They are compatible with both in vitro and in vivo applications, and have been validated with Lipofectamine RNAiMAX Transfection Reagent for use in cell-based systems, and with Invivofectamine 3.0 Transfection Reagent for in vivo delivery. These oligonucleotides are nontoxic and do not induce an immune response in the animal models tested. In vivo-ready mirVana miRNA mimics and inhibitors have been purified by HPLC and dialysis and are ready for immediate in vivo use. For all library inquiries, please email: rnailibraries@lifetech.com.

What is the difference between siRNA and miRNA?

siRNA mediates degradation of a target mRNA molecule in a sequence-specific manner, while miRNA suppresses translation without cleaving mRNA.

What is miRNA?

miRNA (microRNAs) are short, highly conserved small noncoding RNA molecules naturally occurring in the genomes of plants and animals. miRNAs are single stranded, 18-25 nucleotides long and regulate posttranscriptional mRNA expression, typically by binding to the 3' untranslated region (3'-UTR) of the complementary mRNA sequence, resulting in translational repression and gene silencing. Studies have shown that thousands of human protein-coding genes are regulated by miRNAs, indicating that miRNAs are “master regulators” of many important biological processes.

For Research Use Only. Not for use in diagnostic procedures.