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Description
Western blot analysis of PA1-182 detects a ~62 kDa protein in the supernatant of Cypridina luciferase-secreting cells. PA1-182 can be used for activity-independent verification of Cypridina luciferase expression.
The Cypridina luciferase is a 62kDa protein from the marine ostracod, Cypridina noctiluca. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount of Cypridina luciferase protein produced and used to determine the activity of the promoter driving Cypridina expression. Cypridina luciferase can be measured individually or in multiplex assays, such as the Cypridina-Firefly Luciferase Dual Assay Kit (Product # 16183). The Cypridina luciferase has been codon optimized for enhanced performance.
Specifications
| Cypridina luciferase | |
| Polyclonal | |
| Unconjugated | |
| Cypridina noctiluca luciferase | |
| Purified recombinant Cypridina luciferase expressed in E. coli. | |
| 100 μg | |
| Primary | |
| -20°C | |
| Liquid |
| Western Blot | |
| 1 mg/mL | |
| PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide | |
| Rabbit | |
| Protein G | |
| RUO | |
| Luciferase | |
| Antibody | |
| IgG |
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