Invitrogen™ Cytochrome C Monoclonal Antibody (37BA11)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 456100

Catalog No. 45-610-0

Description

45-6100 was used successfully in the western blot analysis of cytochrome C using human breast cancer cell line lysates. Positive control: Isolated mitochondria from Human, Bovine, Rat and Mouse heart; Human fibroblasts; HeLa cells. WB: Predicted molecular weight: 14 kDa. ICC/IF: Heat induced antigen retrieval in 0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C improves signal.

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

Specifications

• Antigen: Cytochrome C
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: HEPES buffered saline with 0.02% sodium azide
• Gene Accession No.: P62894, P62897, P62898, P99999
• Gene Symbols: Cycs
• Immunogen: Bovine heart Cytochrome C.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Bovine, C. elegans
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Clone: 37BA11
• Conjugate: Unconjugated
• Gene: Cycs
• Gene Alias: CG17903; CG17903-PA; CG17903-PB; CRF; CYC; CYC2; Cycs; CYCSA; Cyct-c-p; Cyt c; cyt c-p; cyt.c; CytC; Cyt-c; CYTC2; Cyt-c2; CytC-2; cyt-c-p; cytC-p; Cyt-c-p-PA; Cyt-c-p-PB; Cytochrome c; cytochrome c DC4; Cytochrome c proximal; Cytochrome C, expressed in somatic tissues; cytochrome c, somatic; Cytochrome c-2; cytochrome c-like; cytochrome c-like protein; Cytochrome c-proximal; cytochrome-c-p; Cytochrome-c-proximal; DC4; dCyt.C-p; DMc01; Dmel\CG17903; Dmel_CG17903; hc1; HCS; l(2)k13905; lethal (2) k13905; LOC100341175; LOC100357840; MGC128356 protein; pDMc01; RH17228p; THC4
• Host Species: Mouse
• Purification Method: IgG fraction
• Regulatory Status: RUO
• Gene ID (Entrez): 13063, 25309, 510767, 54205
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov