Invitrogen™ DDX5 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA595091

Catalog No. PIPA595091

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human A431 whole cell, human SH-SY5Y whole cell, human 293T whole cell, rat brain tissue, mouse brain tissue, mouse thymus tissue. IHC: Rat Brain tissue. ICC/IF: A431 cell. Flow: A431 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.

Specifications

• Antigen: DDX5
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DDX5
• Gene Alias: 2600009A06Rik; ATP-dependent RNA helicase DDX5; DDX5; ddx5 gene protein; D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 5; DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 5; DEAD (Asp-Glu-Ala-Asp) box helicase 5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD box protein 5; DEAD box RNA helicase DEAD1; DEAD box-5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); DEAD-box helicase 5; DKFZp434E109; DKFZp686J01190; G17P1; HELR; Hlr1; HUMP68; mDEAD1; MGC118083; p68; p68 RNA helicase; PCR product with interspecies-compatible primers; probable ATP-dependent RNA helicase DDX5; RNA helicase p68; RP23-247J12.4
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13207, 1655, 287765
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 500 μg/mL
• Formulation: PBS with 5mg BSA and 0.05mg sodium azide, 0.05mg thimerosal
• Gene Accession No.: P17844, Q61656
• Gene Symbols: DDX5
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DDX5 (566-580aa TYQNGYDSTQQYGSN).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG