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Invitrogen™ Mouse Decorin (DCN) ELISA Kit

Catalog No. EMDCNX10
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EMDCNX10 10 x 96 Tests
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ELISA

Decorin is a member of the small, leucine-rich proteoglycan (SLRP) family along with biglycan. Found in the interterritorial region of cartilage, decorin interacts with collagen and other matrix proteins to regulate the cartilaginous matrix. It has been shown to interact with fibronectin, thrombospondin, C1q, epidermal growth factor receptor (EGFR), and TGF-b. It is believed that decorin also plays a role in the cell cycle of chondrocytes. The decorin neoepitope fragment has a sequence of PKTLQE, and is generated by MMP-3 or ADAMTS-4 cleavage at Glu154-Leu155 of decorin core protein.
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Specifications

Accession Number P28654
Assay Range 4.1 to 1000 pg/mL
Assay Sensitivity 5 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 13179
Gene Symbol DCN
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Target Decorin
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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