Del-1, Mouse anti-Human, Clone: D345, Millipore Sigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABS197625UG

Catalog No. MABS197625

Description

Detects Del-1 using this Anti-Del-1, clone D345, Cat. No. MABS1976, is a mouse monoclonal antibody that detects Developmentally-regulated endothelial cell locus 1 protein (Del-1) and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Inhibition assay, & Western Blot.

EGF-like repeat and discoidin I-like domain-containing protein 3 (UniProt: O43854; also known as Developmentally-regulated endothelial cell locus 1 protein, Integrin-binding protein DEL1, Del-1) is encoded by the EDIL3 (also known as DEL1) gene (Gene ID: 10085) in human. Del-1 is a calcium-dependent glycoprotein that is secreted by endothelial cells and is shown to be expressed in a developmentally restricted pattern during embryogenesis. In adult tissues it is expressed in endothelial cells and in macrophages. Del-1 is reported to prevent apoptosis in endothelial cell subjected to TNF alpha, etoposide, and anoikis treatment. This anti-apoptotic effect is shown to be mediated specifically through binding of integrin alphav beta3 by the RGD motif. Del-1 expression is reported to be inversely related to that of IL-17 that is shown to inhibit Del-1 via a GSK-3beta and C/EBPb-dependent mechanism. This inhibitory action can be reversed at the PI3K/Akt signaling induced by D-resolvins. It serves as an endothelial cell-secreted homeostatic factor that regulates local inflammation in several tissues. It is reported to interfere with beta2-integrin-dependent inflammatory cell adhesion to endothelium and restricts transendothelial migration of neutrophils. Del-1 is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off in the mature form. It has three EGF-like domains in its N-terminal region. EGF-like domains 2 and 3 form a rigid rod via an interdomain calcium ion binding site, while the long linker between EGF1 and EGF2 lends considerable flexibility to EGF1. Del-1 also has discoidin I-like domains at the C-terminal region that mediate its binding to phosphatidylserine. Two isoforms of Del-1 have been described that are produced by alternative splicing. (Ref.: Dasgupta, SK et al. (2012). Circulation 125 (13); 1664-1672; Wang, Z., et al. (2012). Surgery 151(2); 296-305; Maekawa, T et al. (2015). Nat. Commun. DOI: 10.1038/ncomms9272).

Specifications

• Antigen: Del-1
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal antibody IgG1 in PBS without azide.
• Gene Symbols: EDIL3;DEL1
• Immunogen: GST-tagged full length human recombinant Developmentally-regulated endothelial cell locus 1 protein (Del-1).
• Quantity: 25 μg
• Research Discipline: Apoptosis & Cancer
• Gene ID (Entrez): NP_005702.3
• Target Species: Human
• Form: Purified

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Inhibition Assays, Western Blot
• Clone: D345
• Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Del-1 in human cerebral cortex tissue sections.ELISA Analysis: A representative lot detected Del-1 in ELISA applications (Dasgupta, S.K., et. al. (2012). Ciculation. 125(13):1664-72; Maekawa, T., et. al. (2015). Nat Commun. 6:8272).Inhibition Analysis: A representative lot inhibited the uptake of BODIPY-labeled platelet microparticles by endothelium. (Dasgupta, S.K., et. al. (2012). Ciculation. 125(13):1664-72). Western Blotting Analysis: A representative lot detected Del-1 in Western Blotting applications (Dasgupta, S.K., et. al. (2012). Ciculation. 125(13):1664-72).Flow Cytometry Analysis: A representative lot detected Del-1 in Flow Cytometry applications (Dasgupta, S.K., et. al. (2012). Ciculation. 125(13):1664-72).
• Gene Alias: EGF-like repeat and discoidin I-like domain-containing protein 3;Developmentally-regulated endothelial cell locus 1 protein;Integrin-binding protein DEL1
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone D345 detects human Developmentally-regulated endothelial cell locus 1 protein (Del-1).
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Isotype: IgG1 κ